Development of a real-time PCR target for the detection of Hermetia illucens in feed.

• Marien, A. , Debode, F. , Aerts, C. , Ancion, C. , Francis, F. & Berben, G. (2017). Development of a real-time PCR target for the detection of Hermetia illucens in feed. Jana Pulkrabová, Monika Tomaniová, Michel Nielen and Jana Hajšlová. Proceedings in: 8th International Symposium on Recent Advances in Food Analysis, Prague, Czech Republic, November 7–10, 2017, 342.

Type	Conference Proceedings
Year of conference	2017
Title	Development of a real-time PCR target for the detection of Hermetia illucens in feed.
Editor	Jana Pulkrabová, Monika Tomaniová, Michel Nielen and Jana Hajšlová
Conference name	8th International Symposium on Recent Advances in Food Analysis
Conference location	Prague, Czech Republic
Volume	book of abstracts
Pages	342
Label	U16-conf-Marien-2017-01
conference Date	November 7–10, 2017
Endnote Keywords	insect, Hermetia illucens, black soldier fly, detection, real-time PCR
Abstract	Insects are rich in proteins and could be an alternative source of macronutrients to feed animals and humans. Numerous companies have started the production of insects for feed purposes. In Europe, the processed animal proteins obtained from seven insect species are authorized since 1 st July 2017 for aquaculture by EU regulation 2017/893. Methods of authentication are required to check the conformity of the products. In this study, we propose a real-time PCR method for the specific detection of the black soldier fly (Hermetia illucens L), one of the most widely used insects for feed production. The developed PCR assays amplify a fragment of 67 bp based on the mitochondrial COX3 gene coding for the subunit 3 of the cytochrome C oxidase. The qualitative method was tested according to several performance criteria. The specificity was tested against 50 insect species. The specificity was also checked against plant species and other animal species as crustacean, mammals and birds. The sensitivity, efficiency and robustness of the PCR test were successfully tested. The applicability of the test was proved through the analysis of real-life processed samples (industrial meals) of H. illucens.
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Authors	Marien, A., Debode, F., Aerts, C., Ancion, C., Francis, F., Berben, G.