In vitro screening of durum wheat against water-stress mediated through polyethylene glycol

• Kacem, S. , Delporte, F. , Muhovski, Y. , Djekoun, A. & Watillon, B. (2017). In vitro screening of durum wheat against water-stress mediated through polyethylene glycol. Journal of Genetic Engineering and Biotechnology, 15: (1), 239-247.

Type	Journal Article
Year	2017
Title	In vitro screening of durum wheat against water-stress mediated through polyethylene glycol
Journal	Journal of Genetic Engineering and Biotechnology
Label	U2-Muhovski 2017
Volume	15
Issue	1
Pages	239-247
Date	22 April 2017
Abstract	Three durum wheat (Triticum durum Desf.) genotypes with three levels of drought tolerance were screened in order to evaluate their response to water stress at callus induction and plant regeneration levels. Significant differences were observed among the genotypes, and polyethylene glycol (PEG) levels used, and their interactions were however, significant for all the studied characters. Increase in PEG concentration increased the time required for callus initiation and reduced the number of calli frequency of embryogenic structures and number of plants regenerated, showing the adverse effect of PEG on the somatic embryogenesis developmental., under in vitro conditions tested, and Djenah Khetifa was the most tolerant genotype, followed by Oued Zenati and Waha. This pattern was per their drought tolerance behavior under field conditions. Principal component analysis (PCA) showed that 95.56% of the total variation was explained by the first two principal components. Biplot analysis allowed the stress-tolerant genotype to be distinguished from the two less tolerant genotypes. Time required for callus initiation was strongly negatively correlated with all other studied traits. These traits can be recommended as suitable selection criteria for screening drought-tolerant genotypes. The selected cells and plants will provide a tool for determining the mechanisms involved in tolerance to water stress.
Fichier
Lien	http://dx.doi.org/10.1016/j.jgeb.2017.04.004
Authors	Kacem, S., Delporte, F., Muhovski, Y., Djekoun, A., Watillon, B.