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Classical microscopy: improvements of the qualitative protocol


  • Van Raamsdonk, L. , Veys, P. , Van Cutsem, J. , Pridotkas, G. & Jorgensen, J.S. (2012). Classical microscopy: improvements of the qualitative protocol In: Detection, identification and quantification of processed animal proteins in feedingstuffs, Jorgensen J.S., Baeten V. Namur, Les presses Universitaires de Namur, 47-57.
Type Book Section
Year 2012
Chapter title Classical microscopy: improvements of the qualitative protocol
Book title Detection, identification and quantification of processed animal proteins in feedingstuffs
Editor Jorgensen J.S., Baeten V.
City Namur
Publisher Les presses Universitaires de Namur
Label U15-1618-Veys-2012
Pages 47-57
Isbn 978-2-87551-029-7
Project/Service ref Safeed Pap
Abstract The monitoring of the presence of animal proteins is based on the detection by a microscopic method. The current offi cial description is published in Regulation 152/2009/ EC, which is based on Directive 2003/126/EC. This method is presented here in outline as well as in more detail, also with some recommendations which would provide further optimization. Several additions pertaining to the extraction of muscle fi bres, hair and feather fi laments, handling of fat and oil samples, and spot tests for the detection of specifi c ingredients are presented. Several experiments are discussed describing improvements of the method. A meta-analysis of profi ciency tests indicates that 10 grams of material for starting the sedimentation procedure, and using as much material as possible for examination would optimise the performance of the microscopic method. The latter situation can be achieved by the compulsory examination of the entire sediment using a stereo microscope. A careful choice of an appropriate embedding agent directed to have an optimal viscosity is necessary to optimise the visibility of structures in the particles. The visibility is also infl uenced by applying different colouring methods.
Author address p.veys@cra.wallonie.be
File U15-1618-Veys-2012.pdf
Authors Van Raamsdonk, L. , Veys, P. , Van Cutsem, J. , Pridotkas, G. & Jorgensen, J.S.