Evaluation of three-dimensional gel electrophoresis to improve quantitative profiling of complex proteomes.
- Colignon, B. , Raes, M. , Dieu, M. , Delaive, E. & Mauro, S. (2013). Evaluation of three-dimensional gel electrophoresis to improve quantitative profiling of complex proteomes. Proteomics, 13: 2077-82.
| Type | Journal Article |
| Year | 2013 |
| Title | Evaluation of three-dimensional gel electrophoresis to improve quantitative profiling of complex proteomes. |
| Journal | Proteomics |
| Label | U1 Mauro |
| Volume | 13 |
| Pages | 2077-82 |
| Abstract | Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to alleviate comigration associated drawbacks. Spot resolution is strikingly improved following this simple and rapid method and the positive impact on protein and peptide identification from MS/MS data, on the analysis of relative changes in protein abundance, and on the detection of PTM is described. |
| Fichier | |
| Lien | 10.1002/pmic.201200494 |
| Authors | Colignon, B., Raes, M., Dieu, M., Delaive, E., Mauro, S. |
