Expression of regeneration competence for adventitious buddging, rooting and somatic embryogenesis comparing temperate fruit tree pecies


  • Druart, P. , Ben Mahmoud, K. , Richet, S. & Kondakova, V. (2012). Expression of regeneration competence for adventitious buddging, rooting and somatic embryogenesis comparing temperate fruit tree pecies. Acto Horticulturae (ISHS), 961: 115-121.
Type Journal Article
Year 2012
Title Expression of regeneration competence for adventitious buddging, rooting and somatic embryogenesis comparing temperate fruit tree pecies
Journal Acto Horticulturae (ISHS)
Volume 961
Pages 115-121
Date 2012
Type of article Conference procedings
Isbn 978-906605-595
Endnote keywords In vitro, Totipotence, Compétence à la régénération, cerisier, pommier
Endnote Keywords In vitro culture, Totipotency, somatic embryogenesisn cherry fruit, apple tree
Abstract Fruit trees belong to the recalcitrant species to regeneration. Neo-formed shoots, roots or somatic embryos appear after single culture sequences corresponding to successive phases of induction, initiation and finally elongation (roots), development (buds), or [removed]somatic embryos) according to the organogenic process engaged. Each of the successive steps required specific culture conditions to control according to the genotypes. A comparison has been made between apple, plum, cherry and relevant trees triggering the commonalities between processes of regeneration. Explants must have reached a sufficient physiological maturity before wounding and submitting them to treatments that will activate the perception capacity of the cells to exogenous and single growth regulator at the highest frequency. A gradient of response to the organogenic process highlights more numerous targeted cells in the proximal areas of sub-apical leaves and main roots. The other areas of the explants are involved with optimizing the regeneration conditions. Several cell layers are targeted simultaneously to organize and form new buds and roots on the expression medium. Adventitious budding and somatic embryogenesis can be sustained along subcultures on the expression medium. Through adapted hormonal balance, it is possible to target the layer origin for regenerating buds and the organ for somatic embryos. According to the different levels in root cells competence, various organogenic program simultaneously occur. This comparison shows a similar approach of any regeneration process for any genotype of fruit tree, somatic embryogenesis staying genetic dependent.
Author address druart.p@skynet.be
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Authors Druart, P., Ben Mahmoud, K., Richet, S., Kondakova, V.