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Identification on commercialized products of AFLP markers able to discriminate slow- from fast-growing chicken strains


  • Fumière, O. , Dubois, M. , Grégoire, D. , Théwis, A. & Berben, G. (2003). Identification on commercialized products of AFLP markers able to discriminate slow- from fast-growing chicken strains. J. Agric. Food Chem. 51: 1115-1119.
Type Journal Article
Year 2003
Title Identification on commercialized products of AFLP markers able to discriminate slow- from fast-growing chicken strains
Journal J. Agric. Food Chem.
Label 89
Recnumber 89
Volume 51
Pages 1115-1119
Type of article avec comité de lecture
Endnote keywords RA-CRA-W 2003-2004
Endnote Keywords AFLP|chicken|DNA fingerprinting|authentication|certified productions|slow-growing strains|
Abstract The European chicken meat market is characterized by numerous quality marks: "Label de Qualité Wallon" in Belgium, "Label Rouge" in France, denominations of geographical origin, organic agriculture, etc. Most of those certified productions have specifications requiring the use of slow-growing chicken strains. The amplified fragment length polymorphism (AFLP) technique has been used to search molecular markers able to discriminate slow-growing chicken strains from fast-growing ones and to authenticate certified products. Two pairs of restriction enzymes (EcoRI/MseI and EcoRI/TaqI) and 121 selective primer combinations were tested on individual DNA samples from chicken products essentially in carcass form that were ascribed as belonging to either slow- or fast-growing strains. Within the resulting fingerprints, two fragments were identified as type-strains specific markers. One primer combination gives a band (333 bp) that is specific for slow-growing chickens, and another primer pair generates a band (372 bp) that was found to be characteristic of fast-growing chickens. The two markers were isolated, cloned, and sequenced. The effectiveness and the specificity of the two interesting determinants were assessed on individuals of two well-known strains (ISA 657 and Cobb 500) and on commercialized products coming from various origins.
Author address Fumière Olivier, Quality Department of Agro-food Products, Walloon Agricultural Research Centre (CRA-W), Chaussée de Namur, 24, B-5030 Gembloux, fumiere@cra.wallonie.be
File 272481484989-fumiere-2003.pdf
Caption 89-fumiere-2003.pdf
Link http://pubs.acs.org/cgi-bin/article.cgi/jafcau/2003/51/i05/pdf/jf020748w.pdf
Authors Fumière, O. , Dubois, M. , Grégoire, D. , Théwis, A. & Berben, G.