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Development of real-time PCR targets for the detection of Tenebrio molitor and Hermetia illucens


  • Marien, A. , Debode, F. , Aerts, C. , Ancion, C. , Gérard, A. , Fumière, O. , Francis, F. & Berben, G. (2017). Development of real-time PCR targets for the detection of Tenebrio molitor and Hermetia illucens. Proceedings in: Insectinov2, Romainville-Grand Paris, France, 10-12 October, 40.
Type Conference Proceedings
Year of conference 2017
Title Development of real-time PCR targets for the detection of Tenebrio molitor and Hermetia illucens
Conference name Insectinov2
Conference location Romainville-Grand Paris, France
Volume book of abstracts
Pages 40
Label U16-poster-Marien-2017
conference Date 10-12 October
Endnote Keywords insect, Tenebrio molitor, mealworm, Hermetia illucens, black soldier fly, detection, real-time PCR
Abstract Insects are rich in proteins and could be an alternative source of macronutrients to feed animals and humans. Numerous companies have started the production of insects for feed purposes. In Europe, the processed animal proteins obtained from seven insect species have been authorized since the 1st of July 2017 for aquaculture by EU regulation 2017/893. Methods of authentication are required to check the conformity of the products. In this study, we propose real-time PCR methods for the specific detection of the mealworm (Tenebrio molitor L) and the black soldier fly (Hermetia illucens L), two of the most widely used insects for feed production. Two targets for the detection of T. molitor (94 bp and 114 bp) and one target for the detection of H. illucens (67 bp) are described. These qualitative methods were tested according to several performance criteria. The specificity of each target was tested against a minimum of 34 insect species. The specificity was also checked against plant species and other animal species as crustaceans, mammals and birds. The sensitivity was assessed through the AFNOR XP V03-020-2 standard approach using the LOD6 method. All the methods reached the recommended performance criteria (LOD ? 20 copies). Moreover, for the H. illucens PCR assay, the efficiency and robustness were also successfully tested. The applicability of the tests was proved through the analysis of real-life processed samples (industrial meals).
Author address a.marien@cra.wallonie.be
File Development of real-time PCR targets for the detection of Tenebrio molitor and Hermetia illucens
Authors Marien, A. , Debode, F. , Aerts, C. , Ancion, C. , Gérard, A. , Fumière, O. , Francis, F. & Berben, G.