Lignin in Plant Biomasses: Accuracy of the Detergent Fiber and the Dietary Fiber methods, and Correlation Between these Methods
- Godin, B. , Agneessens, R. , Gerin, P. & Delcarte, J. (2014). Lignin in Plant Biomasses: Accuracy of the Detergent Fiber and the Dietary Fiber methods, and Correlation Between these Methods. Proceedings in: Lignin 2014 - Biosynthesis and utilization, 24-28 August 2014, Umeå, Sweden,
| Type | Conference Proceedings |
| Year of conference | 2014 |
| Title | Lignin in Plant Biomasses: Accuracy of the Detergent Fiber and the Dietary Fiber methods, and Correlation Between these Methods |
| Conference name | Lignin 2014 - Biosynthesis and utilization, 24-28 August 2014, Umeå, Sweden |
| Label | U13-Godin-2014 |
| Abstract | Lignin needs to be quantitated with a high degree of accuracy to estimate well its available amounts and to have a better understanding of its impact on the enzymatic hydrolysis of the cell wall carbohydrates in the rumen and in the cellulosic ethanol process. The detergent fiber (Van Soest) and dietary fiber (sulfuric acid hydrolysis) methods were compared to quantitate the lignin content of plant biomasses based on their accuracy (precision and bias) and on the correlation between these methods. The bias was assessed by a tool established on the basis of the mass balance of the neutral detergent fiber residue (NDF). We showed that the dietary fiber method has the highest degree of accuracy to quantitate the lignin content because of its higher precision and smaller bias, as compared to the detergent fiber method. The tool used to assess the bias also enables to evaluate if the lignin content is not excessively overestimated when the mass balance is above 100%. It showed that the bias of the dietary fiber method for the lignin content was smaller, as compared to detergent fiber method. This bias of the dietary fiber method was smaller because the lignin residue was corrected by its proteins content, the Van Soest acid detergent was not used during the diluted sulfuric acid hydrolysis step and the Van Soest neutral detergent was used to get a first better standardized residue with cellulose, hemicelluloses and lignin before having the lignin residue. Nevertheless, we determined reliable conversion factors to predict the results of the more tedious and resource consuming dietary fiber method by the relatively simple detergent fiber method. Therefore, the detergent fiber method can be used by the biofuel, bio-based chemicals and feed sectors to rank rapidly and accurately plant biomasses. |
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| Authors | Godin, B., Agneessens, R., Gerin, P., Delcarte, J. |
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