Quantification of PAPs in feed by light microscopy : challenge or illusion ?
- Veys, P. , Van Cutsem, J. & Jorgensen, J.S. (2012). Quantification of PAPs in feed by light microscopy : challenge or illusion ? In: Detection, identification and quantification of processed animal proteins in feedingstuffs, Jorgensen J.S., Baeten V. Namur, Les presses Universitaires de Namur, 71-80.
|Chapter title||Quantification of PAPs in feed by light microscopy : challenge or illusion ?|
|Book title||Detection, identification and quantification of processed animal proteins in feedingstuffs|
|Editor||Jorgensen J.S., Baeten V.|
|Publisher||Les presses Universitaires de Namur|
|Project/Service ref||Safeed Pap|
|Abstract||Among the perspectives of the TSE Roadmap, one is to soften, under well defi ned conditions, some aspects of the total ban of animal proteins actually in force. One of these aspects is the possibility of introducing a tolerance level on the presence of fi shmeal in ruminant feeds as it may originate to side-effect cross contamination from fi shmeal containing feeds for non-ruminants. Revising the current feed ban can only start provided adequate control methods are in place for ensuring the correct implementation of a revised feed ban. In this respect, a tolerance level implies a reliable method of quantifi cation for fi sh meal. The sole quantifi cation method is that proposed by Commission Regulation EC 152/2009 which is the offi cial source to apply for the determination of constituents of animal origin in feed by classical light microscopy. Interlaboratory studies have demonstrated some shortages of the quantifi cation method as stated by the directive, and in some cases illustrated its inapplicability. The present chapter aims at presenting the current situation of the quantifi cation method and its shortages and at focusing on potential improvements of the current EC 152/2009 regulation in this matter. Tracks for an optimization of the quantifi cation are developed and commented. Alternative protocols, such as one using classical microscopy but considering only the bone fraction in a feed, under development are also considered and presented. Perspectives but also encountered diffi culties of implementation are discussed.|
|Authors||Veys, P., Van Cutsem, J., Jorgensen, J.S.|