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A set of primers for amplification of mitochondrial DNA in Picea abies and other conifer species


  • Jeandroz, S. , Bastien, D. , Chandelier, A. , Du Jardin, P. & Favre, J. (2002). A set of primers for amplification of mitochondrial DNA in Picea abies and other conifer species. Molecular Ecology Notes, 2: (4), 389-392.
Type Journal Article
Year 2002
Title A set of primers for amplification of mitochondrial DNA in Picea abies and other conifer species
Journal Molecular Ecology Notes
Recnumber 540
Volume 2
Issue 4
Pages 389-392
Date Dec
Endnote Keywords mtDNA, Picea abies, SSCP, PCR-RFLP|inheritance|chloroplast|
Abstract Thirteen primer pairs generating intraspecific length and/or presence-absence polymorphism in Picea abies have been obtained from a P. abies mtDNA library, using different methodologies (agarose gel electrophoresis, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), single-strand conformation (SSCP). Polymorphism tests were extended successfully to other Picea species (P. omorika, P. engelmanii and P. glauca) and species belonging to other conifer genera (Abies alba, Larix laricina and Pinus pinaster). This set of PCR-based mitochondrial markers can provide promising tools for studying phylogeography or phylogeny in P. abies and other conifer species in which the mt genome is generally the only one to be transmitted via the female gamete.
Notes English Article MOL ECOL NOTES 622ZM
Author address Univ Nancy 1, INRA, UMR 1136, F-54506 Vandoeuvre Les Nancy, France; Fac Univ Sci Agron Gembloux, Unite Biol Vegetale, Dept Biochim & Biol Appliquees, B-5030 Gembloux, Belgium FAVRE, JM, UNIV NANCY 1, INRA, UMR 1136, F-54506 VANDOEUVRE LES NANCY, FRANCE fa
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Lien ://000179678400002
Authors Jeandroz, S. , Bastien, D. , Chandelier, A. , Du Jardin, P. & Favre, J.