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01 December 2006
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01 December 2010

SAFEED-PAP

SAFEED-PAP

FP6 EU specific targeted research project, coordinator

Context

Since the earliest case of the bovine spongiform encephalopathy (BSE) epidemic was first confirmed in the United Kingdom in 1987, strict legislation was set up year after year. This has been done taking into account new scientific and epidemiologic evidences showing that meat and bone meal are the most probable vector of the disease, in order to eradicate BSE in Europe. Nowadays, the European Commission has come to the stage that amendments of certain measures could be envisaged, without endangering the health or the policy of eradicating BSE, provided that the positive trend continues and scientific conditions are in place. The scientific conditions to complete regarding the amendments of the total feed ban include the development and validation of analytical methods and tools for the detection of the presence of species-specific animal proteins in animal feed. Actually, the existing methods do not allow species-specific identification and lead to the impossibility to lift the total ban. The SAFEED-PAP project aims to complete the scientific conditions that should allow the repealing of the extended feed ban.

Objectives

The SAFEED-PAP project has three main objectives that should lead to solve the problematic of the species specific detection of animal proteins in compound feeds: (i) Development and validation of suitable methods for the species specific detection and quantification of animal protein in compound feed in order to allow the amendment of the extended total ban; (ii) Development of tools and analytical kits for the correct implementation of the methods in the labs; (iii) To set up the appropriate environment for the optimum application of the methods.

Description of tasks

To achieve these strategic objectives, 7 work packages (WP) have been defined. - WP1 - Coordination and Management of the project - WP2 - Improvement and validation of test kits - WP3 - Development of confirmatory methods using HPLC or MS/MS - WP4 - Development of methods for the quantitative detection and for the species-specific quantitative identification of animal particles by NIRM Microscopy and PCR technics - WP5 - Improvement of the official method and development of a screening method for the control laboratories - WP6 - Validation of methods and feasibility study for the production of certified reference materials - WP7 - Transfer, utilisation and dissemination of results

Contribution

The CRA-W has in charge the coordination and management of the project. The CRA-W is also the work package leader for WP2 and takes part in WP4, WP6 and WP7.

Partners

The SAFEED-PAP consortium coordinated by the CRA-W includes 13 partners (research institutes, universities, private and official control laboratories and SME). The SAFEED-PAP consortium includes European leader research teams involved in the development of analytical methods for the detection of animal proteins and the proper implementation of the method: CRA-W Centre wallon de Recherches agronomiques (Belgium) VLA Veterinary Laboratories Agency (United Kingdom) UCO University of Córdoba (Spain) RIKILT Institute of food safety (The Netherlands) EC-JRC-IRMM European Communities – Directorate General Joint Research Centre – Institute for Reference materials and measurements (Belgium) PDIR Danish Plant Directorate (Denmark) CSL Central Science Laboratory (United Kingdom) FLVVT Federal laboratory for Food and Feed Safety (Belgium) NVL National Veterinary Laboratory of Lithuania (Lithuania) UMIL University of Milan (Italy) CCL Research (The Netherlands) DGO Diagenode S.A (Belgium) CAHEC - China Animal Health and Epidemiology Center (China)

CRAW off coordinator

Dr Vincent Baeten - coordinator CRA-W – Department Quality of Agricultural Products Chaussée de Namur, 24 B 5030 Gembloux Tel : +32 (0)81 62 03 50 Fax : +32 (0)81 62 03 88 Email : baeten@cra.wallonie.be Website: http://safeedpap.feedsafety.org/

Funding

  • CE - DG Research - FP6

Publications

Fumière, O. , Close, R. , Karanam, M. & Berben, G. (2012). DNA based approaches for the species-specific identification of animal material In: Detection, identification and quantification of processed animal proteins in feedingstuffs, Namur, Les presses Universitaires de Namur, 93-102. Bremer, M. , Fumière, O. , Hekman, W. , Marien, A. , Kemmers-Vonken, A. , Fernández Pierna, J.A. , Vliege, J. , Baeten, V. , Van Raamsdonk, L. & Berben, G. (2012). Combination methods for PAP detection and species determination of animal particles In: Detection, identification and quantification of processed animal proteins in feedingstuffs, Jorgensen J.S., Baeten V. Namur, Les presses Universitaires de Namur, 139-149. Fernández Pierna, J.A. , Baeten, V. , Boix, A. , Von Holst, C. , Perez Marin, D. & Garrido Varo, A. (2012). Near Infrared microscopy (NIRM) In: Detection, identification and quantification of processed animal proteins in feedingstuffs, Jorgensen J.S., Baeten V. Namur, Les presses Universitaires de Namur, 81-91. Veys, P. , Van Cutsem, J. & Jorgensen, J.S. (2012). Quantification of PAPs in feed by light microscopy : challenge or illusion ? In: Detection, identification and quantification of processed animal proteins in feedingstuffs, Jorgensen J.S., Baeten V. Namur, Les presses Universitaires de Namur, 71-80. Vermeulen, P. , Von Holst, C. , Boix, A. & Baeten, V. (2012). Animal proteins in feed: legislative aspects and analytical consequences In: Detection, identification and quantification of processed animal proteins in feedingstuffs, Jorgensen J.S., Baeten V. Namur, Les presses Universitaires de Namur, 23-34. FUMIèRE, O. (2007). Developpment of alternative methods for the detection of PAPs in feedingstuffs. Proceedings in: 1st CRL-AP Workshop, Gembloux - Belgique, Abbas, O. , Fernández Pierna, J.A. , Boix, A. , Von Holst, C. , Dardenne, P. & Baeten, V. (2010). Key parameters for the development of a NIR Microscopic method for the quantification of processed by-product of animal origin in compound feedingstuffs. Anal. Bioanal. Chem. 397: (5), 1965-1973. Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2010). In-house validation of a near infrared hyperspectral imaging method for detecting processed animal proteins (PAP) in compound feed. Journal of NIRS, 18: 121-133. Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2009). Development of a hyperspectral NIR imaging to detect animal species in compound feeds. Poster in: 3rd International Feed Safety Conference 2009: Methods and Challenges, Wageningen - The Netherlands, 6-7 October 2009. Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2009). A near infrared (NIR) hyperspectral imaging system running under accreditation ISO 17025 for the detection of Meat and Bone Meal (MBM) in compound feed. Poster in: International workshop on multivariate image analysis, Valencia - Spain, 28-29 September 2009. Fernández Pierna, J.A. , Baeten, V. , Dardenne, P. & Fernández Pierna, J.A. (2008). A backward variable selection method for PLS regression (BVSPLS). Poster in: 11th International Conference on Chemometrics in Analytical Chemistry (CAC 2008), Monpellier - France, 29 June - 4 July 2008. Abbas, O. , Baeten, V. , Dardenne, P. & Fernández Pierna, J.A. (2009). A Backward Variable Selection method for PLS regression (BVSPLS). Anal. Chim. Acta, 642: (1-2), 89-93. Von Holst, C. , Baeten, V. , Boix, A. , Slowikowsky, B. , Tirendi, S. , Dardenne, P. & Fernández Pierna, J.A. (2008). Transferability study of a near-infrared microscopic method for the detection of banned meat and bone meal feedingstuffs. Anal. Bioanal. Chem. 392: 313-317. Boix, A. , Baeten, V. , Slowikowsky, B. , Von Holst, C. , Tirendi, S. , Dardenne, P. & Fernández Pierna, J.A. (2007). Transferability study of a Near-Infrared Microscopic method for the detection of banned Meat and Bone Meal in feedinfstuffs. Poster in: 13th International Conference on Near Infrared Spectroscopy, Umea - Sweden, 15-21 June 2007. Van Raamsdonk, L. , Von Holst, C. , Baeten, V. , Berben, G. , Boix, A. & De Jong, J. (2007). New developments in the detection and identification of processed animal proteins in feeds. Anim. Feed Sci. Technol. 133: 63-83. Fumière, O. , Marien, A. , Fernández Pierna, J. , Baeten, V. & Berben, G. (2010). Development of a real-time PCR protocol for the species origin confirmation of isolated animal particles detected by NIRM. Food addit. Contam. 27: (8), 1118-1127.

Team

Vincent BAETEN Gilbert BERBEN Juan Antonio FERNANDEZ PIERNA Olivier FUMIERE Philippe VERMEULEN Aline MARIEN Hervé NOEL
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