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Characterization of alder Phytophthora isolates from Wallonia and development of SCAR primers for their specific detection


  • Merlier, D. , Chandelier, A. , Debruxelles, N. , Noldus, M. , Laurent, F. , Dufays, E. , Claessens, H. & Cavelier, M. (2005). Characterization of alder Phytophthora isolates from Wallonia and development of SCAR primers for their specific detection. Journal of Phytopathology, 153: (2),
Type Journal Article
Year 2005
Title Characterization of alder Phytophthora isolates from Wallonia and development of SCAR primers for their specific detection
Journal Journal of Phytopathology
Recnumber 19
Volume 153
Issue 2
Pages 99-107
Date Feb
Endnote Keywords assays|detection|fungal diseases|pathogenicity|plant diseases|plant pathogenic fungi|plant pathogens|techniques|Phytophthora alni|
Abstract Isolates of alder Phytophthora were collected in the southern part of Belgium on riverbanks planted with Alnus glutinosa and A. incana. They were compared with strains isolated in other European countries in terms of maximum temperature for growth, oogonia shape, pathogenicity on Alnus seedlings and genetic traits. Using both molecular techniques [random amplified polymorphic DNA (RAPD) and random amplified microsatellite (RAMS)], two groups of isolates were identified, the first group being further divided into two subgroups, Ia and Ib, using RAPD. Most of the Walloon alder Phytophthora isolates as well as the standard type from UK (formally designated P. alni subsp. alni) fell into group Ia. One isolate was classified in group Ib with the German and Dutch variants (P. alni subsp. multiformis), while three isolates were placed with the Swedish variant (P. alni subsp. uniformis) in group II. In terms of morphological properties, isolates from groups Ia and Ib developed colonies with a felt-like appearance and usually produced numerous oogonia, varying from wavy to warty after 1 week (group Ia) or 2-3 weeks (Ib) in darkness. In contrast, colonies from group II isolates were generally irregular, and smooth oogonia were produced in low quantities after approximately 1 month in culture. A polymerase chain reaction (PCR) using sequence-characterized amplification region (SCAR) primers derived from a polymorphic amplification product generated with a RAPD primer was developed for the specific detection of alder Phytophthora. The specificity and sensitivity of this test are discussed here.
Notes Cited Reference Count: 25 ref. Journal article English
Author address Department of Biological Control and Plant Genetic Resources, Walloon Agricultural Research Centre, Rue de Liroux, 4, 5030 Gembloux, Belgium.
Lien ://20053029511
Authors Merlier, D. , Chandelier, A. , Debruxelles, N. , Noldus, M. , Laurent, F. , Dufays, E. , Claessens, H. & Cavelier, M.