Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays


  • Chandelier, A. , Helson, M. , Dvorak, M. & Gisher, F. (2014). Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays. Plant Pathology, Doi: 10.1111/ppa.12218.
Type Journal Article
Year 2014
Title Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays
Journal Plant Pathology
Label U3 - Mycologie
Edition Journal Article
Recnumber 20
Volume 63
Pages Doi: 10.1111/ppa.12218
Endnote Keywords ascospore, Hymenoscyphus pseudoalbidus, invasive pathogen, real-time PCR, spore trap
Abstract A method based on real-time polymerase chain reaction (PCR) and the use of rotating-arm spore traps was developed for quantifying airborne Hymenoscyphus pseudoalbidus ascospores. The method was sensitive and reproducible, and the collection efficiency was 10% of the spores present in the air. The temporal ascospore dispersal pattern was studied over 3 years by collecting spores every 15 days for a 24 h air-sampling period during the ash-growing season. The highest production was detected from the end of June to the beginning of September. The overall ascospore production did not differ significantly among stands within a specific year but there were differences from year to year. There was a positive correlation between air temperature and the number of ascospores trapped, with most of the positive samples being observed at temperatures above 12°C. The vertical profile of ascospore dispersal showed a strong decrease in ascospore density within a height of 3 m, regardless of date of collection. An analysis of the spore traps installed at increasing distances from an infected stand showed that most of the ascospores were deposited downwind within 50 m of the stand. These data are discussed in context of the epidemiology of the disease
Authors Chandelier, A. , Helson, M. , Dvorak, M. & Gisher, F.