Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays
- Chandelier, A. , Helson, M. , Dvorak, M. & Gisher, F. (2014). Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays. Plant Pathology, 63: Doi: 10.1111/ppa.12218.
|Title||Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays|
|Label||U3 - Mycologie|
|Endnote Keywords||ascospore, Hymenoscyphus pseudoalbidus, invasive pathogen, real-time PCR, spore trap|
|Abstract||A method based on real-time polymerase chain reaction (PCR) and the use of rotating-arm spore traps was developed for quantifying airborne Hymenoscyphus pseudoalbidus ascospores. The method was sensitive and reproducible, and the collection efficiency was 10% of the spores present in the air. The temporal ascospore dispersal pattern was studied over 3 years by collecting spores every 15 days for a 24 h air-sampling period during the ash-growing season. The highest production was detected from the end of June to the beginning of September. The overall ascospore production did not differ significantly among stands within a specific year but there were differences from year to year. There was a positive correlation between air temperature and the number of ascospores trapped, with most of the positive samples being observed at temperatures above 12°C. The vertical profile of ascospore dispersal showed a strong decrease in ascospore density within a height of 3 m, regardless of date of collection. An analysis of the spore traps installed at increasing distances from an infected stand showed that most of the ascospores were deposited downwind within 50 m of the stand. These data are discussed in context of the epidemiology of the disease|
|Authors||Chandelier, A., Helson, M., Dvorak, M., Gisher, F.|