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Detection of Barley yellow mosaic virus and Barley mild mosaic virus by ELISA and RT-PCR: a comparison study


  • Steyer, S. , Barbier, A. , Denis, F. , Collard, M. , Vaïanopoulos, C. & Bragard, C. (2005). Detection of Barley yellow mosaic virus and Barley mild mosaic virus by ELISA and RT-PCR: a comparison study. Parasitica, 61: (1), 33-36.
Type Journal Article
Year 2005
Title Detection of Barley yellow mosaic virus and Barley mild mosaic virus by ELISA and RT-PCR: a comparison study
Journal Parasitica
Recnumber 678
Volume 61
Issue 1
Pages 33-36
Type of article PSR
Isbn 0031-1812
Abstract Two bymoviruses responsible for systemic mosaic symptoms on barley, Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) are present in most of the cereal soils of Belgium, causing yield losses in susceptible varieties. Symptoms may appear at the end of December but are evident in early spring. They tend to disappear as the weather becomes warmer. The control of the soil-borne mosaic disease is mainly managed by the use of resistant varieties. The resistance is traditionally assessed on the basis of the symptom expression or ELISA performed on the leaves. A broad-spectrum RT-PCR was developed targeting the conserved 3'-end of virus RNA-1. A comparison is made between the results obtained with the two different detection methods.
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Authors Steyer, S. , Barbier, A. , Denis, F. , Collard, M. , Vaïanopoulos, C. & Bragard, C.