Detection of Barley yellow mosaic virus and Barley mild mosaic virus by ELISA and RT-PCR: a comparison study
- Steyer, S. , Barbier, A. , Denis, F. , Collard, M. , Vaïanopoulos, C. & Bragard, C. (2005). Detection of Barley yellow mosaic virus and Barley mild mosaic virus by ELISA and RT-PCR: a comparison study. Parasitica, 61: (1), 33-36.
| Type | Journal Article |
| Year | 2005 |
| Title | Detection of Barley yellow mosaic virus and Barley mild mosaic virus by ELISA and RT-PCR: a comparison study |
| Journal | Parasitica |
| Recnumber | 678 |
| Volume | 61 |
| Issue | 1 |
| Pages | 33-36 |
| Type of article | PSR |
| Isbn | 0031-1812 |
| Abstract | Two bymoviruses responsible for systemic mosaic symptoms on barley, Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV) are present in most of the cereal soils of Belgium, causing yield losses in susceptible varieties. Symptoms may appear at the end of December but are evident in early spring. They tend to disappear as the weather becomes warmer. The control of the soil-borne mosaic disease is mainly managed by the use of resistant varieties. The resistance is traditionally assessed on the basis of the symptom expression or ELISA performed on the leaves. A broad-spectrum RT-PCR was developed targeting the conserved 3'-end of virus RNA-1. A comparison is made between the results obtained with the two different detection methods. |
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| Caption | \\Server\users\Commun\Publications, Bibliothèque\Publications D3\Virologie |
| Authors | Steyer, S., Barbier, A., Denis, F., Collard, M., Vaïanopoulos, C., Bragard, C. |
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