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Evaluation of barley yellow dwarf virus tolerance: a controlled field system compared with a molecular marker test


  • Steyer, S. , Leal, C. , Gregoire, D. & Froidmont, D. (1999). Evaluation of barley yellow dwarf virus tolerance: a controlled field system compared with a molecular marker test. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, 106: (5),
Type Journal Article
Year 1999
Title Evaluation of barley yellow dwarf virus tolerance: a controlled field system compared with a molecular marker test
Journal Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
Recnumber 9
Volume 106
Issue 5
Pages 553-555
Date Sep
Endnote Keywords barley|ELISA|polymerase chain reaction|plant diseases|plant pathogens|disease resistance|testing|molecular biology|cereals|plant pathology|Luteoviridae|
Abstract For the evaluation of BYDV tolerance, a controlled field system was developed to test barley lines from a breeding programme. ELISA tests confirmed the presence of the virus in the plants. Visual assessments were made during the spring to select tolerant F4 generations. A PCR marker was used to discriminate between Yd2 and non-Yd2 barley plants. In parallel trials, plants were analysed for the presence of the Yd2 gene. Results obtained with the controlled field systems were compared with the PCR test. The cost and time taken were evaluated for each method and the breeding possibilities offered with the combined methods are discussed.
Notes Cited Reference Count: 3 ref. Journal article English
Author address Centre de Recherches Agronomiques, Departement de Lutte Biologique et de Ressources Phytogenetiques, Chemin de Liroux, B-5030 Gembloux, Belgium.
Lien ://19991007900
Authors Steyer, S. , Leal, C. , Gregoire, D. & Froidmont, D.