Evolution and distribution of hydrolytis enzyme activities during preharvest sprouting of wheat (Triticum aestivum) in the field.


  • Olaerts, H. , Roye, C. , Derde, L. , Sinnaeve, G. , Meza, R. , Bodson, B. & Courtin, C. (2016). Evolution and distribution of hydrolytis enzyme activities during preharvest sprouting of wheat (Triticum aestivum) in the field. Journal of agricultural and food chemistry, 64: 5644-5652.
Type Journal Article
Year 2016
Title Evolution and distribution of hydrolytis enzyme activities during preharvest sprouting of wheat (Triticum aestivum) in the field.
Journal Journal of agricultural and food chemistry
Label U14_2016
Volume 64
Pages 5644-5652
Endnote keywords wheat, amylase activity, sprout damaged
Abstract To date, research on preharvest sprouted (PHS) wheat has mostly been conducted on kernels germinated under laboratory conditions, which differ widely from conditions in the field. To obtain detailed knowledge of the evolution of hydrolytic enzyme activities in PHS wheat (Triticum aestivum), a broad collection of samples from three varieties was obtained by harvesting before, at, and after maturity. Delaying harvest time coupled with periods of heavy rainfall caused sprouting in the kernels, observed as a drop in Falling Number and an increase in ?-amylase activity. The appearance of ?- and ?-amylase, peptidase, and endoxylanase activity during field sprouting was independent from each other. Consequently, Falling Number could not be used to predict activity of other hydrolytic enzymes. When differentiating endogenous from kernel-associated microbial enzymes, results showed that ?- and ?-amylase and peptidase activity of PHS kernels were predominantly of endogenous origin, whereas endoxylanase activity was largely from microbial origin.
Author address g.sinnaeve@cra.wallonie.be
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Authors Olaerts, H., Roye, C., Derde, L., Sinnaeve, G., Meza, R., Bodson, B., Courtin, C.