• Suivez-nous

Genetic and molecular characterization of Verticillium dahliae isolates from woody ornamentals in Belgian nurseries


  • Chandelier, A. , Laurent, F. , Dantinne, D. , Mariage, L. , Etienne, M. & Cavelier, M. (2003). Genetic and molecular characterization of Verticillium dahliae isolates from woody ornamentals in Belgian nurseries. European Journal of Plant Pathology, 109: (9), 943-952.
Type Journal Article
Year 2003
Title Genetic and molecular characterization of Verticillium dahliae isolates from woody ornamentals in Belgian nurseries
Journal European Journal of Plant Pathology
Recnumber 533
Volume 109
Issue 9
Pages 943-952
Date Nov
Endnote Keywords Acer spp., PCR, Tilia spp., VCG, Verticillium wilt|vegetative compatibility groups|cotton|differentiation|strains|rapd|pcr|susceptibility|pathogenicity|reassessment|virulence|
Abstract Isolates of Verticillium dahliae were collected from affected trees (Acer spp., Tilia spp. and Robinia spp.) and soils in Belgian ornamental nurseries. Nitrate non-utilizing mutants were produced and vegetative compatibility groups (VCGs) were classified based on complementation tests with reference tester strains. Of the 30 isolates analysed, 12 were classified as VCG2B and 18 as VCG4B following the American classification. In order to distinguish VCG2B from VCG4B, specific polymerase chain reaction primers were designed based on the sequence of a VCG2B-associated Direct Amplification of Minisatellite-region DNA ( DAMD) band generated with the core sequence of the phage M13 minisatellite DNA. Using this test, amplification products were generated for all the VCG2B isolates characterized in this study. In contrast, no signal was seen on ethidium-bromide agarose gel for VCG4B isolates. Pathogenicity tests were carried out in a glasshouse on maple-rooted cuttings inoculated with conidial suspensions of V. dahliae belonging to both groups (VCG2B/VCG4B). Some strains proved to be highly aggressive, while others did not. However, these different behaviours were not correlated with the VCGs.
Notes English Article EUR J PLANT PATHOLOGY 744CW
Author address Agr Res Ctr, Dept Biol Control & Plant Genet Resources, B-5030 Gembloux, Belgium CHANDELIER, A, AGR RES CTR, DEPT BIOL CONTROL & PLANT GENET RESOURCES, RUE LIROUX 4, B-5030 GEMBLOUX, BELGIUM chandelier@cra.wallonie.be
Fichier
Lien ://000186611200007
Authors Chandelier, A. , Laurent, F. , Dantinne, D. , Mariage, L. , Etienne, M. & Cavelier, M.