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Genetic diversity among European cultivated spelt revealed by microsatellites


  • Bertin, P. , Gregoire, D. , Massart, S. & De Froidmont, D. (2001). Genetic diversity among European cultivated spelt revealed by microsatellites. Theoretical and Applied Genetics, 102: (1), 148-156.
Type Journal Article
Year 2001
Title Genetic diversity among European cultivated spelt revealed by microsatellites
Journal Theoretical and Applied Genetics
Recnumber 528
Volume 102
Issue 1
Pages 148-156
Date Jan
Endnote Keywords spelt, wheat, SSR, microsatellite, genetic diversity|triticum-aestivum l|gel-electrophoretic analyses|quantitative trait loci|winter-wheat cultivars|seed storage proteins|bread wheat|l forms|markers|population|distance|
Abstract Spelt and common wheat constitute two of the six groups of the hexaploid wheats with an AA-BBDD genome. Spelt culture has been progressively replaced by that of common wheat which out-yields spelt under high-input conditions. In the last decades, spelt breeders intended to introduce the yield-potential and bread-making qualities of common wheat into spelt, by frequent crossings between accessions of these two different groups. The present study aims at determining the genetic basis of modern spelt cultivars in terms of intra-group variability and inter-group (spelt vs common wheat) distances, by using microsatellite markers developed for common wheat. The allelic composition of 30 spelt and nine common wheat accessions was determined at 17 microsatellite loci. The coefficient of co-ancestry (f) and the genetic distances (1 - proportion of shared alleles) based upon allelic composition were calculated for all pairs of accessions. Two dendrograms were constructed using the UPGMA method. Amplification products were found for all loci on most accessions. A total of 113 alleles was identified, of which 60.2% were specific to spelt or common wheat. The correlation between (1 - f) and the genetic distance was high (0.701***). The mean pairwise genetic distance was 0.656 +/- 0.181 over the 39 accessions, 0.706 +/- 0.14 among common wheat and 0.573 +/- 0.172 among spelt. The mean genetic distance between spelt and wheat was 0.782 +/- 0.113. The two dendrograms were in accordance with each other and clearly separated the spelt from the common wheat accessions. It is concluded that microsatellites developed for common wheat and distances based on the proportion of shared alleles are powerful tools for reconstructing phylogenies in spelt, and that the genetic basis of modern spelt cultivars is narrow despite frequent crosses made with bread wheat.
Notes English Article THEOR APPL GENET 399TU
Author address Ctr Rech Agron Etat, Dept Lutte Biol & Ressources Phytogenet, B-5030 Gembloux, Belgium BERTIN, P, UNIV CATHOLIQUE LOUVAIN, DEPT BIOL APPL & PROD AGRICOLES, UNITE ECOL GRANDES CULTURES, PL CROIX DU SUD,2 BTE 11, B-1348 LOUVAIN LA NEUVE, BELGIUM
Fichier
Lien ://000166829100019
Authors Bertin, P. , Gregoire, D. , Massart, S. & De Froidmont, D.