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High level of genetic diversity among spelt germplasm revealed by microsatellite markers


  • Bertin, P. , Gregoire, D. , Massart, S. & De Froidmont, D. (2004). High level of genetic diversity among spelt germplasm revealed by microsatellite markers. Genome, 47: (6), 1043-1052.
Type Journal Article
Year 2004
Title High level of genetic diversity among spelt germplasm revealed by microsatellite markers
Journal Genome
Recnumber 530
Volume 47
Issue 6
Pages 1043-1052
Date Dec
Endnote Keywords spelt, SSR, micro satellites, genetic diversity, germplasm|triticum-aestivum l|bread wheat|aegilops-tauschii|yellow rust|d genome|resistance|map|
Abstract The genetic diversity of spelt (Triticum aestivum (L.) Thell. subsp. spelta (L.) Thell.) cultivated presently is very narrow. Although the germplasm collections of spelt are extensive, the related genetic knowledge is often lacking and makes their use for genetic improvement difficult. The genetic diversity and structure of the spelt gene pool held in gene banks was determined using 19 simple sequence repeat (SSR) markers applied to 170 spelt accessions collected from 27 countries and 4 continents. The genetic distances (1-proportion of shared alleles) were calculated and an unweighted pair-group method with arithmetic averaging (UPGMA)-based dendrogram was generated. The genetic diversity was high: 259 alleles were found and the mean interaccession genetic distance was 0.782 +/- 0.141. The dendrogram demonstrated the much higher genetic diversity of spelt held in germplasm collections than in the currently used genotypes. Accessions with the same geographical origin often tended to cluster together. Those from the Middle East were isolated first. All but one of the Spanish accessions were found in a unique subcluster. Most accessions from eastern Europe clustered together, while those from northwestern Europe were divided into two subclusters. The accessions from Africa and North America were not separated from the European ones. This analysis demonstrates the extent of genetic diversity of spelts held in germplasm collections and should help to widen the genetic basis of cultivated spelt in future breeding programs.
Notes English Article GENOME 896IH
Author address Univ Catholique Louvain, Dept Biol Appliquee & Prod Agricoles Ecol Grandes, B-1348 Louvain, Belgium; Ctr Rech Agron, Dept Lutte Biol & Ressources Phytogenet, B-5030 Gembloux, Belgium BERTIN, P, UNIV CATHOLIQUE LOUVAIN, DEPT BIOL APPLIQUEE & PROD AGRICOLES
Fichier
Lien ://000226927300006
Authors Bertin, P. , Gregoire, D. , Massart, S. & De Froidmont, D.