Identification and validation of Arabidopsis thaliana SUMO5-interacting proteins
- Muhovski, Y. , Antoine, A. , Gonzalez, E. , Berendzen, K. , Twizere, J.C. & Mauro, S. (2016). Identification and validation of Arabidopsis thaliana SUMO5-interacting proteins. Poster in: Belgian Proteomics Association conference (BePA): "Exploring the hidden depths of the proteome", University Aula in Ghent, Belgium, 17-18 November.
| Type | Poster |
| Year | 2016 |
| Title | Identification and validation of Arabidopsis thaliana SUMO5-interacting proteins |
| Event name | Belgian Proteomics Association conference (BePA): "Exploring the hidden depths of the proteome" |
| Event location | University Aula in Ghent, Belgium |
| Label | U2- Muhovski -2016 |
| Event date | 17-18 November |
| Abstract | In eukaryotes, posttranslational modification by the addition of Small Ubiquitin-like Modifier (SUMO) alters the activity of many substrate proteins. First, this regulatory mechanism was described in yeast and mammals where it is implicated in various cellular processes such as chromatin modifications, RNA transport, transcriptional regulation, cell development, interaction with pathogens… Protein SUMOylation is an enzymatic process with several well characterized players: SUMO protease, SUMO-activating and -conjugating enzymes, ligase and deSUMOylating isopeptidase. In Arabidopsis, it has been shown that disruption of this pathway is lethal, highlighting the significance of SUMOylation in plant development. Despite this, little is known about SUMO targets in plants where the number of SUMO genes and their activity vary across species. Most of the studies have been performed in Arabidopsis. Interestingly, in its genome eight SUMO paralogs have been identified but only four (1, 2, 3 and 5) showed time- and condition-dependent expression. In the present study, we focused on identification and validation of Arabidopsis SUMO5 targets by yeast two-hybrid system as a primary identification method coupled with in planta validation using bimolecular fluorescence complementation and flow cytometry approach. In summary, we identified 212 SUMO5 targets and their in planta validation is on the way. |
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| Authors | Muhovski, Y., Antoine, A., Gonzalez, E., Berendzen, K., Twizere, J.C., Mauro, S. |
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