Identification of interactions between Phytophthora infestans RxLR effectors and Solanum tuberosum host proteins by yeast two-hybrid approach


  • Muhovski, Y. & Rolot, J. (2015). Identification of interactions between Phytophthora infestans RxLR effectors and Solanum tuberosum host proteins by yeast two-hybrid approach. Poster in: EAPR Breeding and Varietal assessement section/ EUCARPIA Section potatoes, Vico Equense, Italy, 15-18 November 2015.
Type Poster
Year 2015
Title Identification of interactions between Phytophthora infestans RxLR effectors and Solanum tuberosum host proteins by yeast two-hybrid approach
Event name EAPR Breeding and Varietal assessement section/ EUCARPIA Section potatoes
Event location Vico Equense, Italy
Label U2
Event date 15-18 November 2015
Abstract Potato (Solanum tuberosum L.) is the third most important food crop just after wheat and rice and as each crop it is challenged by various pathogens. Lacking an adaptive immune system, plants including potato have developed a monitoring system to detect and divert pathogen invasions. Defense responses can be initiated on the host cell surface by so called pattern recognition receptors which detect particular conserved molecular signatures of pathogens or apoplastic effector proteins. In turn, pathogens deliver other effector proteins that operate within host cells and interfere with pattern recognition receptor immunity in order to promote disease progression. Effectors can be recognized by plant disease resistance (R) proteins but little is known about the identities of the host proteins that are targeted by oomycete effectors and even less is known about the roles those targets play in plant immunity. One of the most devastating potato diseases is the late blight caused by the oomycete Phytophthora infestans. According to genome information, the pathogen secrets more than 500 RxLR effectors that are translocated inside the host cells. The goal of our study is to gain a better insight into the function of P. infestans RxLR proteins. For that purpose, yeast 2-hybrid (Y2H) approach was applied to identify candidate host targets of P. infestans RxLR effectors. Y2H cDNA library from potato infected with the pathogen was constructed and screened with candidate RxLR molecules. Our experiments aim to learn more about the regulation of expression and the delivery system of RxLR-effectors and to identify their molecular targets in the host. In conclusion, a more in depth knowledge of RxLR biology is central for understanding and ultimately controlling diseases caused by Phytophthora.
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Authors Muhovski, Y. & Rolot, J.