Induction of rhizogenesis of in vitro culture of cuttings of Argania spinosa (L).Skeels

  • Alaoui, M. . , Gaboun, F. , Benbya, A. , Farhoune, H. , Chlyah, O. , Delporte, F. & Cherkaoui, S. (2018). Induction of rhizogenesis of in vitro culture of cuttings of Argania spinosa (L).Skeels. In Vitro Developmental Cellular Biology - Plant. s38-s83.
Type Conference Proceedings
Year of conference 2018
Title Induction of rhizogenesis of in vitro culture of cuttings of Argania spinosa (L).Skeels
Editor In Vitro Developmental Cellular Biology - Plant
Volume 54 (suppl 1)
Pages s38-s83
Abstract The argan tree (Argania spinosa (L.) Skeels), of the Sapotaceae family is an endemic species and is emblematic of the south-western Moroccan flora. It occupies the third place in forest species and covers an area of 828000 ha. Deeply anchored in the daily life of rural populations, it plays a fundamental role in their livelihood. The excessive harvest of its seeds for food, cosmetic and medicinal virtues led to the degradation of the Moroccan argan tree. The economic, social and environmental importance of the argan tree and its derivatives implies the necessity and importance of accelerating the restoration of the forest by replanting argan trees. The present work aims at optimizing the rooting conditions of vitroplants resulting from in vitro micro-cuttings. An in vitro micropropagation protocol from woody cuttings has been developed through previous studies. Axillary bud burst and stem elongation were achieved on modified Lloyd and Mc Cown’s Woody Plant medium supplemented with combinations of three plant growth regulators: 6-benzylaminopurine (BAP), kinetin (Kin) and gibberellic acid (GA 3). The explants produced tufts of shoots, an average of 5.4 ± 1 per explant. The subcultures were performed every 4 to 6 weeks before transplanting the vitroplants into the rooting medium. For the rooting phase, we used half-diluted Murashige and Skoog (MS) medium with 1 mg L-1 BAP added. The study was focused on the effect on the rhizogenic potential of four concentration (0.5; 2.5; 5 and 10) mg L-1 of three auxins AIA, ANA, or AIB. The results showed that AIA intake has a low rooting rate of no more than 5% but a better root count, giving an average of 6.5 ± 1 roots per plantlet. However, AIB and ANA had rooting rates of 25% and 12.5% respectively, but with an average number of 3.4 roots per plantlet.
Authors Alaoui, M. ., Gaboun, F., Benbya, A., Farhoune, H., Chlyah, O., Delporte, F., Cherkaoui, S.