Interactions between Phytophthora infestans RxLR effectors and potato host proteins

  • Muhovski, Y. & Rolot, J. (2014). Interactions between Phytophthora infestans RxLR effectors and potato host proteins. Poster in: 19th Triennial Conference EAPR 2014, Brussels, Belgium, 6 > 11 July 2014.
Type Poster
Year 2014
Title Interactions between Phytophthora infestans RxLR effectors and potato host proteins
Event name 19th Triennial Conference EAPR 2014
Event location Brussels, Belgium
Label U2-Muhovski 2014
Event date 6 > 11 July 2014
Abstract Potato (Solanum tuberosum) is the third most important food crop just after wheat and rice. As each crop, potato suffers from different diseases caused by various pathogens. Lacking an adaptive immune system, plants including potato have evolved two-tier monitoring system to detect and deflect pathogen invasion. These two levels of inducible plant defense provide hurdles to infection. Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) follows the perception of conserved microbial molecules at the surface of plant cells. Plant pathogens secrete effector proteins that suppress PTI. Effectors may be recognized by plant disease resistance (R) proteins, resulting in effector-triggered immunity (ETI) often involving hypersensitive response (a form of programmed cell death) coinciding with restriction of the invading pathogen. Thus, many pathogen effectors have evolved to suppress the programmed cell death as a component of either PTI or ETI. One of the most devastating potato diseases is caused by the oomycete Phytophthora infestans. The pathogen secrets RxLR effectors that are translocated inside the host cells [1,2]. The conserved motif RxLR found in these effectors is needed for their translocation. The number of RxLR effector genes in Phytophthora genome is remarkably high, with about 563 predicted in P. infestans [3]. Although sequence information, the little that is known about the manipulation of plant targets by oomycete effectors is focused on those RxLR which are recognized by plant resistance proteins. The goal of our recently started project is to gain a better insight into the function of RxLR-effectors using the interaction of potato with Phytophthora infestans. To identify candidate host targets of P. infestans RxLR effectors, a yeast-two-hybrid library composed of cDNA from potato infected with the pathogen is under construction and screening with candidate RxLR effector molecules. Our experiments aim to learn more about the regulation of expression and the delivery system of RxLR-effectors and to identify their molecular targets in the host plant. In conclusion, a more in depth knowledge of RxLR-effector biology is central for understanding and ultimately controlling diseases caused by Phytophthora. [1] Vleeshouwers V et al., 2011. Understanding and exploiting late blight resistance in the age of effectors. Annu Rev Phytopathol 49, 25.1-25.25 [2] Bos J et al., 2010. Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1. PNAS 107, 9909-9914 [3] Haas BJ et al., 2009. Genome sequence and analysis of the Iris potato famine pathogen Phytophthora infestans. Nature 461, 393-398
Authors Muhovski, Y., Rolot, J.