Proteomics analysis of the endogenous, constitutive, leaf SUMOylome


  • Colignon, B. , Delaive, E. , Dieu, M. , Demazy, C.. , Muhovski, Y. , Wallon, C.. , Raes, M. & Mauro, S. (2016). Proteomics analysis of the endogenous, constitutive, leaf SUMOylome. Journal of Proteomics,
Type Journal Article
Year 2016
Title Proteomics analysis of the endogenous, constitutive, leaf SUMOylome
Journal Journal of Proteomics
Endnote keywords protéomique
Abstract SUMOylation is a post-translational modification which regulates a number of critical biological processes in, for example mammals, yeast and plants. In order to fully understand the functional effects of SUMOylation an essential first step is the identification of endogenous targets for SUMOylation. Here we report the results of using a recently developed proteomic approach based on the use of 3D gels to identify the endogenous SUMO targets in leaves of Solanum tuberosum. By using 3D gels we avoid the problem of co-migration of proteins, which is a major limitation of 2D gels, and we enable the use of the highly sensitive CyDye DIGE fluor saturation dyes. Using this new method we have identified 39 individual proteins as probable SUMO targets in leaves of Solanum tuberosum. The advantages of this method compared with other approaches are discussed, and possible future developments are outlined.
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Lien http://dx.doi.org/10.?1016/?j.?jprot.?2016.?09.?012
Authors Colignon, B., Delaive, E., Dieu, M., Demazy, C.., Muhovski, Y., Wallon, C.., Raes, M., Mauro, S.