Transcriptomics as a tool for identification of potato genotypes associating pathogen resistance and high efficiency of nitrogen utilization. Model: Solanum tuberosum x Phytophthora infestans x nitrogen


  • Muhovski, Y. , Antoine, A. , Gonzalez, E. & Mauro, S. (2019). Transcriptomics as a tool for identification of potato genotypes associating pathogen resistance and high efficiency of nitrogen utilization. Model: Solanum tuberosum x Phytophthora infestans x nitrogen. Poster in: When biology meets oxygen and other matters, University of Namur, Namur, 13/09/2019.
Type Poster
Year 2019
Event name When biology meets oxygen and other matters
Event location University of Namur, Namur
Label U1 Muhovski
Event date 13/09/2019
Project/Service ref FIRST
Endnote Keywords RNA-seq, potato, P. infestans, nitrogen
Abstract Potato (Solanum tuberosum L.) is the third most important food crop just after wheat and rice and as each crop, it is not immunized against the negative impact of various biotic and abiotic challenges like pathogens and over fertilization. Lacking an adaptive immune system, plants including potato developed a monitoring system to detect-divert or simply to give up to the pathogen invasions. Late blight caused by the oomycete Phytophthora infestans is one of the most devastating potato diseases and often 15 to 18 fungicide treatments per growing season are needed to fight the pest. Therefore, using resistant varieties is more practical way regarding ecology and economy in order to control the disease. On general, most of the potato varietal selection programs are disease-resistance orientated and important cultivar feature like nitrogen (N) use efficiency is often neglected. Excessive loss of nitrate from potato root zone is a serious environmental issue worldwide so developing of potato cultivars which utilize N more efficiently is a long-term alternative in reducing such a loss. Combining these two major biotic and abiotic issues and RNA-seq, a whole transcriptome analysis, based on next-generation sequencing we try to depict mechanisms for resistance/sensitivity and better N utilization in potato genotypes. To achieve that goal, two potato genotypes with contrasting resistance to late blight were grown hydroponically in growth chamber on three different NO3? concentrations (1.5, 4 and 15mM) and infected with 2 P. infestans strains with distinct virulence. The infection was monitored by measuring the expression of specific pathogen markers using qRT-PCR and samples were collected after 0, 6, 16, 48 and 72h for time-course RNA-sequencing analysis.
Fichier RNA-seq.pdf
Authors Muhovski, Y. , Antoine, A. , Gonzalez, E. & Mauro, S.