Use of 16S rRNA V1 région analysis for the identification of lactobacilli occurring in a kefir grain
- Ninane, V. , Mukandayambaje, R. & Berben, G. (2006). Use of 16S rRNA V1 région analysis for the identification of lactobacilli occurring in a kefir grain. Proceedings in: 2nd IDF dairy science and technology week - Scientific and technological challenges in fermented milk, Sirmione - Italy, 15-19/05/2006, FIL-IDF,
| Type | Conference Proceedings |
| Year of conference | 2006 |
| Title | Use of 16S rRNA V1 région analysis for the identification of lactobacilli occurring in a kefir grain |
| Conference name | 2nd IDF dairy science and technology week - Scientific and technological challenges in fermented milk |
| Conference location | Sirmione - Italy |
| Publisher | FIL-IDF |
| Recnumber | 906 |
| Label | 906 |
| conference Date | 15-19/05/2006 |
| Abstract | The aim of this work was to verify the applicability of partial 16S rRNA analysis to the identification of lactobacilli in the complex microflora of a kefir grain (the KJ consortium). Sequencing a part of the 16S rRNA is more convenient than sequencing the whole molecule that is nowadays a taxonomy tool largely recognized. Moreover, some particular regions of 16S rRNA seem to be more interesting than the whole molecule for the identification of microorganisms: differences in 16S rRNA between various microorganisms are not equally distributed in the whole molecule, but concentrated in localized regions called variable regions [1]. Among these, the variable V1 region was successfully used in the development of molecular methods for the detection and the identification of isolated lactobacilli species [2, 3]. The originality of the method applied to the kefir grain, is that isolation of lactobacilli by growing on selective culture media was avoided. Fragments analysed were indeed amplified from the DNA directly extracted from the kefir grain and thereafter differentiated by cloning in Escherichia coli. Based on the similarity of their V1 regions, which were of about 60 base pairs length, sequences from 83 fragments were pooled into three groups. They were corresponding to Lactobacillus kefiranofaciens, Lb. kefir and Lb. parakefir, when aligned with the sequences of a library [4]. This molecular identification was verified by classical biochemical methods used in microbiology. [1] Neefs J.M. et al. (1993). Compilation of small ribosomial subunit RNA structures. Nucleic Acids Res. 21, 3025-3049. [2] Kullen M.J. et al. (2000). Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex. J. Appl. Microbiol. 89, 511-516. [3] Tärnberg M. et al. (2002). Identification of randomly selected colonies of Lactobacilli from normal vaginal fluid by pyrosequencing of the 16S rDNA variable V1 and V3 regions. Acta Pathol. Microbiol. Immunol. Scand. 802-810. [4] Pearson W.R. and Lipman D.J. (1988). Improved tools for biological sequence analysis. Proc. Natl. Acad. Sci. USA 85, 2444-2448. |
| Author address | Ninane Véronique, Quality Department of Agro-food Products, Walloon Agricultural Research Centre (CRA-W), Chaussée de Namur, 24, B-5030 Gembloux, ninane@cra.wallonie.be |
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| Caption | 906-ninane-2006.doc |
| Authors | Ninane, V., Mukandayambaje, R., Berben, G. |
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