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Use of VIRAZOLE(R) to eradicate odontoglossum ringspot virus from in vitro cultures of Cymbidium Sw


  • Toussaint, A. , Kummert, J. , Maroquin, C. , Lebrun, A. & Roggemans, J. (1993). Use of VIRAZOLE(R) to eradicate odontoglossum ringspot virus from in vitro cultures of Cymbidium Sw. Plant Cell, Tissue and Organ Culture, 32: (3),
Type Journal Article
Year 1993
Title Use of VIRAZOLE(R) to eradicate odontoglossum ringspot virus from in vitro cultures of Cymbidium Sw
Journal Plant Cell, Tissue and Organ Culture
Recnumber 81
Volume 32
Issue 3
Endnote Keywords Techniques|Ribavirin|control|disease control|in vitro culture|ornamental plants|plant pathology|elimination|
Abstract Odontoglossum ringspot tobamovirus (ORV) was eradicated from Cymbidium by chemotherapy based on incorporation of ribavirin (as VIRAZOLE) into the in vitro culture medium of protocorms. Applications of the virustatic agent for several consecutive subcultures freed protocorms of the virus. Acclimatized plantlets regenerated from these protocorms were healthy as determined by ELISA. No resurgence of virus occurred over a period of 5 years. Trueness to type was total for colour and morphology of flowers and the batch of plants grown on was perfectly homogeneous. To secure fast and effective eradication of the virus during the consecutive subcultures of protocorms with ribavirin, 3 factors were important: accurate isolation of new growths from initial tissues, ribavirin concn and frequency of transplanting in new media.
Notes Cited Reference Count: 21 ref. Journal article English
Author address Unite d'Enseignement et de Recherche des Cultures Ornementales, Faculte des Sciences Agronomiques de Gembloux, Avenue de la Faculte d'Agronomie 2, 5030 Gembloux, Belgium.
Fichier
Lien ://19932336492
Authors Toussaint, A. , Kummert, J. , Maroquin, C. , Lebrun, A. & Roggemans, J.