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Widespread occurrence of Wheat spindle streak mosaic virus in Belgium


  • Vaïanopoulos, C. , Legrève, A. , Lorca, C. , Moreau, V. , Steyer, S. , Maraite, H. & Bragard, C. (2006). Widespread occurrence of Wheat spindle streak mosaic virus in Belgium. Plant Disease, 90: (6), 723-728.
Type Journal Article
Year 2006
Title Widespread occurrence of Wheat spindle streak mosaic virus in Belgium
Journal Plant Disease
Recnumber 682
Volume 90
Issue 6
Pages 723-728
Type of article PSR
Endnote Keywords Polymyxa graminis|soilborne viruses|
Abstract In order to assess the occurrence of Wheat spindle stread mosaic virus (WSSMV) in Belgium, a reverse-transcription polymerase chain reation (RT-PCR) was developed, targeting WSSMV isolates from Canada, France, Italy, and the United States. The primers also were designed for virus quantification by real-time RT-PCR with SYBR-green. No cross-reaction with soilborne cereal viruses such as mild mosaic virus, Barley yellow mosaic virus, soil-borne cereal mosaic virus, and Soil-borne wheat mosaic virus was observed. The RT-PCR and real-time quantitative RT-PCR allowed a more sensitive detection of WSSMV than enzyme-linked immunosorbent assay. The incidence of WSSMV in Belgium was evaluated using a bio-presence of WSSMV was a detected from plants grown in 32% of the soils. The RT-PCR methods developed here, combined with large sampling, allowed WSSMV to be detected for the first time in Belgium. The real-time quantitative RT-PCR was developed as a tool for evaluating the resistance to WSSMV by quantifying the virus concentration in wheat cultivars.
Notes Impact Factor : 1.479
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Authors Vaïanopoulos, C. , Legrève, A. , Lorca, C. , Moreau, V. , Steyer, S. , Maraite, H. & Bragard, C.