Widespread occurrence of Wheat spindle streak mosaic virus in Belgium
- Vaïanopoulos, C. , Legrève, A. , Lorca, C. , Moreau, V. , Steyer, S. , Maraite, H. & Bragard, C. (2006). Widespread occurrence of Wheat spindle streak mosaic virus in Belgium. Plant Disease, 90: (6), 723-728.
|Title||Widespread occurrence of Wheat spindle streak mosaic virus in Belgium|
|Type of article||PSR|
|Endnote Keywords||Polymyxa graminis|soilborne viruses||
|Abstract||In order to assess the occurrence of Wheat spindle stread mosaic virus (WSSMV) in Belgium, a reverse-transcription polymerase chain reation (RT-PCR) was developed, targeting WSSMV isolates from Canada, France, Italy, and the United States. The primers also were designed for virus quantification by real-time RT-PCR with SYBR-green. No cross-reaction with soilborne cereal viruses such as mild mosaic virus, Barley yellow mosaic virus, soil-borne cereal mosaic virus, and Soil-borne wheat mosaic virus was observed. The RT-PCR and real-time quantitative RT-PCR allowed a more sensitive detection of WSSMV than enzyme-linked immunosorbent assay. The incidence of WSSMV in Belgium was evaluated using a bio-presence of WSSMV was a detected from plants grown in 32% of the soils. The RT-PCR methods developed here, combined with large sampling, allowed WSSMV to be detected for the first time in Belgium. The real-time quantitative RT-PCR was developed as a tool for evaluating the resistance to WSSMV by quantifying the virus concentration in wheat cultivars.|
|Notes||Impact Factor : 1.479|
|Authors||Vaïanopoulos, C. , Legrève, A. , Lorca, C. , Moreau, V. , Steyer, S. , Maraite, H. & Bragard, C.|