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Combination methods for PAP detection and species determination of animal particles


  • Bremer, M. , Fumière, O. , Hekman, W. , Marien, A. , Kemmers-Vonken, A. , Fernández Pierna, J.A. , Vliege, J. , Baeten, V. , Van Raamsdonk, L. & Berben, G. (2012). Combination methods for PAP detection and species determination of animal particles In: Detection, identification and quantification of processed animal proteins in feedingstuffs, Jorgensen J.S., Baeten V. Namur, Les presses Universitaires de Namur, 139-149.
Type Book Section
Year 2012
Chapter title Combination methods for PAP detection and species determination of animal particles
Book title Detection, identification and quantification of processed animal proteins in feedingstuffs
Editor Jorgensen J.S., Baeten V.
City Namur
Publisher Les presses Universitaires de Namur
Label U15-1622-Fernandez-2012
Pages 139-149
Isbn 978-2-87551-029-7
Project/Service ref Safeed Pap
Abstract Two novel methods for the detection of processed animal proteins based on a combination of optical microscopy with immunochemical detection techniques, and on a combination of Near Infrared Microscopy (NIRM) with polymerase chain reaction (PCR) are presented. These combinations of methods add the possibility of obtaining species information on individual particles at a low level of detection. Such an on the spot identifi cation of microscopically identifi ed particles with respect to the source (species) would strongly enhance the support of the legislation, especially the species-tospecies ban. In this chapter experimental details on sample treatment and clean-up of individual particles as well as optimisation of detection procedures for both combinations of methods will be discussed in particular.
Author address fernandez@cra.wallonie.be
File Combination methods for PAP detection and species determination of animal particles
Authors Bremer, M. , Fumière, O. , Hekman, W. , Marien, A. , Kemmers-Vonken, A. , Fernández Pierna, J.A. , Vliege, J. , Baeten, V. , Van Raamsdonk, L. & Berben, G.