cDNA-AFLP Analysis of Differentially Expressed Transcripts in Partially Resistant Apple Cultivar Leaves Infected by Venturia inaequalis


  • Bastiaanse, H. , Pissard, A. , Mingeot, D. , Muhovski, Y. , Jacquemin, J-M. , Lepoivre, P. & Lateur, M. (2013). cDNA-AFLP Analysis of Differentially Expressed Transcripts in Partially Resistant Apple Cultivar Leaves Infected by Venturia inaequalis In: Acta Hortica, K.M.Evans et als. Leuven-Belgium, ISHS, 976, 513-519.
Type Book Section
Year 2013
Chapter title cDNA-AFLP Analysis of Differentially Expressed Transcripts in Partially Resistant Apple Cultivar Leaves Infected by Venturia inaequalis
Book title Acta Hortica
Editor K.M.Evans et als.
City Leuven-Belgium
Publisher ISHS
Edition Eucarpia
Volume 976
Pages 513-519
Project/Service ref Proc.of the 13th Eucarpia Symposium on Fruit Breeding and Genetic
Endnote keywords apple, President Roulin, cDNA-AFLP, partial scab resistance analysis
Abstract Apple scab is one of the most important diseases of apple. Although resistance gene governing qualitative resistance have been isolated and characterized, the biological roles fo genes governing quantitative resistance are stil unknown. This study investigated the molecular and biochemical mechanisms involved in the partial resistance against Venturia inaequalis of an old Belgian apple cultivar 'Président Roulin'. Gene expression in both resistant and susceptible apple cultivars after scab inoculation was studied using the cDNA-AFLP method. From about 10,250 cDNA fragments detected, 252 bands were up- or down-regulated in the resistant cultivar 'Gala'. Some 224 fragments were constituvely expressed by the resistant cultivar only, and were not affected by the fungal infection. All the pathogen- responsive fragments and some of the fragments showing contitutive expression were cloned, sequenced and compared with GENBANK accessions using blastx. Homologies to known genes revealed several proteins belonging to the NBS-LRR and LRR-RLK classe of plant R genes. Other defense-related transcripts were also identified and could play a role in a partial scab defense response. Expression of these genes was verified by real-time quantitative PCR.
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Authors Bastiaanse, H. , Pissard, A. , Mingeot, D. , Muhovski, Y. , Jacquemin, J-M. , Lepoivre, P. & Lateur, M.