Detection of Ruminant Meat and Bone Meals in Animal Feed by Real-Time Polymerase Chain Reaction: Result of an Interlaboratory Study
- Prado, M. , Berben, G. , Fumière, O. , Van Duijn, G. , Mensinga-Kruize, J. , Reaney, S. , Boix, A. & Von Holst, C. (2007). Detection of Ruminant Meat and Bone Meals in Animal Feed by Real-Time Polymerase Chain Reaction: Result of an Interlaboratory Study. J. Agric. Food Chem. 55: (18), 7495-7501.
|Title||Detection of Ruminant Meat and Bone Meals in Animal Feed by Real-Time Polymerase Chain Reaction: Result of an Interlaboratory Study|
|Journal||J. Agric. Food Chem.|
|Endnote Keywords||ruminant, beef, feed, animal meals, MBM, mitochondrial/chromosomal DNA|Real time PCR||
|Abstract||The commercialization of animal feeds infected by prions proved to be the main cause of transmission of bovine spongiform encephalopathy (BSE). Therefore, feed bans were enforced, initially for reminant feeds, and later for all feeds for farmed animal. The development and validation of analytical methods for the species-specific detection of animal proteins in animal feed has been indicated in the TSE (Transmissible Spongiform Encephalopathies) Roadmap (European Comission. The TSE (Transmissible Spongiform Encephalopathy) roadmap. URL: http://europa.eu.int/comm/food/food/biosafety/bse/roadmap_en.pdf, 2005) as the main condition for lifting the extended feed ban. Methods base on polymerase chain reaction (PCR) seem to be a promising solution for this aim. The main objective of this study was to determine the applicbility of four different rea-time PCR methods, developed by three National expert laboratories from the European Union (Eu), for the detection and identification of cattle or ruminant species in typical compound feeds, fortifies with meat and bone meals (MBM) from different animal species at different concentration levels. The MBM samples utilized in this study have been treated using the sterilization condition mandatory within the European Union (steam pressure sterilization at 133°C, 3 bar, and 20 min), which is an additional challenge to the PCR methods evaluated in this study. The results indicate that the three labs applying their PCR methods were able to detect 0.1% of cattle MBM, either alone or in mixtures with different materials such as fishmeal, which demonstrates the improvement made by this technique, especially when compared with results from former interaloratory studies.|
|Author address||Berben Gilbert, Quality Department of Agro-food Products, Walloon Agricultural Research Centre (CRA-W), Chaussée de Namur, 24, B-5030 Gembloux, email@example.com|
|Authors||Prado, M., Berben, G., Fumière, O., Van Duijn, G., Mensinga-Kruize, J., Reaney, S., Boix, A., Von Holst, C.|