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Development of a real-time PCR protocol for the species origin confirmation of isolated animal particles detected by NIRM


  • Fumière, O. , Marien, A. , Fernández Pierna, J. , Baeten, V. & Berben, G. (2010). Development of a real-time PCR protocol for the species origin confirmation of isolated animal particles detected by NIRM. Food addit. Contam. 27: (8), 1118-1127.
Type Journal Article
Year 2010
Title Development of a real-time PCR protocol for the species origin confirmation of isolated animal particles detected by NIRM
Journal Food addit. Contam.
Label 678
Recnumber 678
Volume 27
Issue 8
Pages 1118-1127
Date 8 août 2010
Type of article scientifique
Isbn 0265-203X print1464-5122 online
Endnote Keywords authenticity|molecular biology|polymerase chain reaction|PCR|animal products|animal feedingstuffs|animal feed|
Abstract At present, European legislation prohibits totally the use of processed animal proteins in feed for all farmed animals (Commission Regulation (EC) No. 1234/2003 ? extended feed ban). A softening of the feed ban for non-ruminants would nevertheless be considered if alternative methods could be used to gain more information concerning the species origin of processed animal proteins than that which can be provided by classical optical microscopy. This would allow control provisions such as the ban of feeding animals with proteins from the same species or intra-species recycling (Regulation (EC) No. 1774/2002). Two promising alternative methods, near-infrared microscopy (NIRM) and real-time polymerase chain reaction (PCR), were combined to authenticate, at the species level, the presence of animal particles. The paper describes the improvements of the real-time PCR method made to the DNA extraction
Notes DOI: 10.1080/19440049.2010.481639
Author address Fumière Olivier, Quality Department of Valorisation Products, Walloon Agricultural Research Centre (CRA-W), Chaussée de Namur, 24, B-5030 Gembloux, fumiere@cra.wallonie.be
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Authors Fumière, O. , Marien, A. , Fernández Pierna, J. , Baeten, V. & Berben, G.