Profiles of the volatile organic compounds emitted by the masses of Abies nordmanniana somatic embryos at maintenance and maturation stages.


  • Druart, P. , Michiels, F. , Misson, J.P. , Seffer, X. , Wathelet, J.P. & Fauconnier, M.L. (2013). Profiles of the volatile organic compounds emitted by the masses of Abies nordmanniana somatic embryos at maintenance and maturation stages. 02/07.
Type Poster
Year 2013
Title Profiles of the volatile organic compounds emitted by the masses of Abies nordmanniana somatic embryos at maintenance and maturation stages.
Event date 02/07
Abstract Somatic embryogenesis is the only way to propagate well-shaped Abies nordmanniana clonally. It would also be the most promising technology for breeding such woody species without genetic re-combinations. As Christmas tree, the aromatic trait is a priority and the lines putatively improved require a precocious identification. That is the reason why the emissions of the volatiles organic compounds have been considered at the most important stages of the in vitro process: the maintenance of immature embryos masses and their maturation, respectively. The masses of immature embryos were grown on maintenance culture medium before their transfer to different maturation media. Two weeks after growth, the volatile organic compounds (VOCs) accumulating in the head-space of in vitro culture jars were trapped in sterile nondestructive quantitative conditions on tenax ®columns. The VOCs were desorbed from the adsorbent in a thermodesorption unit and then separated by gas chromatography on a polar column. The identification of the VOCs was performed by mass spectrometry and by comparisons with mass spectra libraries and calculated retention indexes to the one of literature. The embryos masses emitting VOCs as a function of culture media were compared on the base of total VOCs emission and individual compound relative percentages. Total VOCs emission was clearly influenced by the composition of the culture medium especially when masses were transferred from maintenance to maturation stage. The individual compound relative percentages were also impacted by the transfer of one culture medium to another.
Fichier Profiles of the volatile organic compounds emitted by the masses of Abies nordmanniana somatic embryos at maintenance and maturation stages.
Authors Druart, P., Michiels, F., Misson, J.P., Seffer, X., Wathelet, J.P., Fauconnier, M.L.