Suitability of ruminant identification assays for use in processed animal proteins and animal feed

  • Van Den Hoven, S. , Vaessen, J. , Margry, R. , Fumière, O. , Berben, G. & Baeten, V. (2007). Suitability of ruminant identification assays for use in processed animal proteins and animal feed. CRA-W. Proceedings in: FEED SAFETY International Conference 2007: Methods and Challenges, Namur - Belgique, 27-28/11/2007, 74-75.
Type Conference Proceedings
Year of conference 2007
Title Suitability of ruminant identification assays for use in processed animal proteins and animal feed
Editor CRA-W
Conference name FEED SAFETY International Conference 2007: Methods and Challenges
Conference location Namur - Belgique
Recnumber 1100
Pages 74-75
Label U15-1100
conference Date 27-28/11/2007
Endnote Keywords Species identification intra-species recycling ban|PCR|immunochemical assay|reference materials|
Abstract As a preventive measure to avoid the spread of Bovine Spongiform Encephalopathy (BSE), a feed ban had been introduced by the European Union (EC Regulation 999/2001). The Animal By-Product Regulation (EC) No.1774/2002 allows the use of products from category 3 materials for inclusion in feed, but this is subjected to strict rules, including an intra-species recycling ban. It is prohibited to feed animals with processed animal proteins (PAP s) from the same species. Detection and identification of animal tissues in feed has therefore gained great interest. At present, microscopy is still the only official method for detection of PAP s in feed in the European Union. Besides microscopy there is a need for a technique which can discriminate between species and is less time consuming. The availability of an analytical technique for species identification would facilitate the lift of the feed ban. The suitability of several ruminant, porcine and poultry identification assays is tested with different reference material. Some of the reference material (porcine soft material, porcine bone material, chicken soft material and chicken bone material) is processed by CCL in a dedicated steriliser under strict conditions (20 minutes at 133 C or 159 C; both pre- and postpressure cooked). Other reference material (bovine, ovine, porcine and avian carcase and muscle material) is processed by PDM Ltd (Doncaster, UK) at 133 C, 137 C, 141 C and 145 C. Furthermore, some pure commercial available products are used as reference material. With these reference materials several mixtures are prepared, both in PAP and feed. One immunochemical and four PCR assays of different research institutes, that claim to be able to identify ruminant species, were tested with the reference material and the mixtures of this reference material. Another four PCR assays, which claim to be able to identify porcine and poultry species were also tested. The immunochemical and one of the PCR assays were carried out by CCL. The other assays were carried out by the institute, which developed them. The results show that the assays seem quite suitable for species identification, even after heating for twenty minutes at the highest temperatures. However, some of the ruminant assays do not always reach the preferred detection limit of 0.1%. It is still desirable to reduce the number of false positive and negative results and test some of the assays with more reference samples. In general, the results show that these assays are quite promising. This will help acceptance of porcine and poultry PAP s in aquafeed and to make it possible again to feed porcine PAP s to poultry and poultry PAP s to pigs.
Author address Fumière Olivier, Quality Department of Agro-food Products, Walloon Agricultural Research Centre (CRA-W), Chaussée de Namur, 24, B-5030 Gembloux,
Caption U15-1100-fumiere-2007.pdf
Authors Van Den Hoven, S., Vaessen, J., Margry, R., Fumière, O., Berben, G., Baeten, V.