Development of a hyperspectral NIR imaging to detect animal species in compound feeds
- Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2009). Development of a hyperspectral NIR imaging to detect animal species in compound feeds. Poster in: 3rd International Feed Safety Conference 2009: Methods and Challenges, Wageningen - The Netherlands, 6-7 October 2009.
|Title||Development of a hyperspectral NIR imaging to detect animal species in compound feeds|
|Event name||3rd International Feed Safety Conference 2009: Methods and Challenges|
|Event location||Wageningen - The Netherlands|
|Event date||6-7 October 2009|
|Abstract||One of the main concerns of the CRA-W is the development and the validation of new methods for a rapid, precise and reliable detection of meat and bone meal (MBM) in feedstuffs. For this reason, we proposed the use of the Near infrared microscopy (NIRM) and the Near Infrared hyperspectral imaging methods as promising alternative methods having clear advantages (speed, flexibility, easy to use?). NIR imaging technique consists in the analysis of several hundreds of particles being the result of the grinding of compound feedstuffs. The major advantages are that the recognition is independent on the expertise of the analyst and that it is possible to automate all procedures and to analyse more samples per unit of time than classical microscopy. A complete strategy has been developed in order to discriminate feed particles according to the species. This strategy comprises a complete experimental design, improvements in the acquisition step (sample preparation and sample stage and software) as well as a complete chemometric and statistic procedure. Chemometric classification strategies have been applied to automate the method, and reduce the need for constant expert analysis of the data. Multivariate classification methods as Support Vector Machines (SVM) or Partial Least Squares Discriminant Analysis (PLS-DA) have been used in the procedure for the classification of feed particles as the different species using the NIR hyperspectral images. This strategy has driven us, in the last years, to different studies and publications concerning this problematic as the use of the hyperspectral NIR technique for a complete screening of compound feeds or a fully study in order to include the technique in the framework of the accreditation under ISO 17025. The method is running under accreditation ISO 17025 since 2005 at the CRA-W and is used in the activities of the Community Reference Laboratory for Animal Proteins (CRL-AP).|
|Authors||Dardenne, P. , Baeten, V. & Fernández Pierna, J.A.|