Detection of ergot bodies in cereals by hyperspectral near infrared imaging

  • Vermeulen, P. , Dardenne, P. , Baeten, V. & Fernández Pierna, J.A. (2009). Detection of ergot bodies in cereals by hyperspectral near infrared imaging. Poster in: International workshop on multivariate image analysis, Valencia - Spain, 28-29 September 2009.
Type Poster
Year 2009
Title Detection of ergot bodies in cereals by hyperspectral near infrared imaging
Event name International workshop on multivariate image analysis
Event location Valencia - Spain
Label U15-1454
Recnumber 627
Event date 28-29 September 2009
Endnote Keywords feed safety|ergot|kernel analysis|NIR hyperspectral imaging|
Abstract Contamination of cereals with ergot, formed by the fungi Claviceps purpurea is well known. For the farmer, the damage caused by ergot is a yield reduction: the ergot replaces the kernels in the grain ears. For the feed/food sector, the presence of ergot in feedingstuffs and agro-food products involves high toxicity risk for animal and human in relation to the alkaloid composition and content in the ergot. The neurotoxic signs comprise feed/food refusal, dizziness but also convulsions. A survey on the presence of undesirable botanic substances in feed, carried out in 2006 inside official control labs from all member states of the European Union, showed a resurgence of the ergot presence in cereals samples. To reduce the risk of poisoning, the European directive 2002/32/EC on undesirable substances in animal feed fixed a limit in the EU of 0.1% for ergot in all feedingstuffs containing unground cereals. The Commission is preparing regulations for ergot alkaloids in food and feed, but methods of analysis are still lacking. No limits have been set for individual alkaloids as yet. The existing microscopy method provides an elegant early warning tool for ergot contamination but is time-consuming. The current work, performed in the framework of the CONffIDENCE project, aims to assess by hyperspectral NIR imaging the presence of ergot bodies in cereals. For this experiment, a total of 85 ergot bodies issue from different sources (Belgium, The Netherlands, Germany and Denmark) and 100 wheat and barley kernels issue from several varieties and Belgian locations have been collected and analyzed with a MatrixNIRTM Chemical Imaging System (Malvern instruments Ltd). This instrument is active in the 900- 1700 nm range and allows to collect the NIR spectra of 10 kernels in 5 minutes. A total of around 3000 spectra from each kernel is obtained. This study showed the potential of hyperspectral NIR imaging to discriminate the ergot bodies from barley and wheat kernels. Additional developments will be undertaken for the quantification of ergot bodies in the samples. Further research will be also carried out in order to develop and validate a method allowing the on-line detection of ergot bodies in cereals.
Caption U15-1454-vermeulen-2009.pdf
Authors Vermeulen, P., Dardenne, P., Baeten, V., Fernández Pierna, J.A.